| 產品編號 | bs-5884R-BF647 |
| 英文名稱 | Rabbit Anti-Endomucin/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的內皮粘蛋白EMCN抗體 |
| 別 名 | Endomucin 2; Mucin 14; EMCN; EMCN2; Endomucin; Endomucin-2; Endothelial sialomucin; Gastric cancer antigen Ga34; MUC-14; MUCEN_HUMAN; Mucin-14. |
| 規(guī)格價格 | 50ug/1680元 購買 100ug/2980元 購買 大包裝/詢價 |
| 說 明 書 | 50ug 100ug |
| 研究領域 | 腫瘤 免疫學 信號轉導 糖蛋白 細胞骨架 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, (predicted: Rat, Dog, Cow, ) |
| 產品應用 | Flow-Cyt=1ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 25kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Endomucin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. Function: Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. Subcellular Location: Secreted and Cell membrane. Membrane. Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks. Tissue Specificity: Expressed in heart, kidney and lung. Post-translational modifications: Highly O-glycosylated. Sialic acid-rich glycoprotein. Database links: Entrez Gene: 51705 Human Omim: 608350 Human SwissProt: Q9ULC0 Human Unigene: 152913 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-Endomucin/AF647 Conjugated antibody (bs-5884R-BF647)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-AF647 .
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
