| 產(chǎn)品編號(hào) | bs-60075R |
| 英文名稱 | Phospho-Histone H3 (Thr45) Rabbit pAb |
| 中文名稱 | |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | Antigen affinity purification |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 組蛋白翻譯后修飾（PTMs）是表觀遺傳學(xué)調(diào)控染色質(zhì)結(jié)構(gòu)的關(guān)鍵機(jī)制，被稱為“組蛋白密碼”。組蛋白上的翻譯后修飾包括乙?；?，甲基化，磷酸化和近年發(fā)現(xiàn)的一些新型酰化修飾。這些組蛋白修飾直接影響染色質(zhì)和轉(zhuǎn)錄因子或其他表觀調(diào)控子的結(jié)合，改變基因組的穩(wěn)定性和基因轉(zhuǎn)錄等。組蛋白磷酸化通常發(fā)生在核心組蛋白N端的絲氨酸，蘇氨酸或酪氨酸上，在DNA修復(fù)，轉(zhuǎn)錄和染色質(zhì)折疊等方面起著重要作用。廣為人知的組蛋白磷酸化位點(diǎn)為H2A.xS139ph，有文獻(xiàn)報(bào)道此位點(diǎn)與DNA損傷相關(guān)。組蛋白磷酸化主要參與有絲分裂和減數(shù)分裂過(guò)程。參與調(diào)控磷酸化水平的激酶與磷酸酶多種多樣。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 17 kDa
Observed MW: 17 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) JK, (+) JK+ TPA (200nM, 30min) + Calyculin A (100nM, 1hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 17 kDa
Observed MW: 17 kDa
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