| 產(chǎn)品編號 | bsm-52869R |
| 英文名稱 | CD90/Thy-1 Recombinant Rabbit mAb |
| 中文名稱 | CD90重組兔單抗 |
| 別 名 | CD90/Thy1; CD7; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; T |
| 研究領域 | 細胞生物 免疫學 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 4C6 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:1000-5000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD90/Thy-1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation. Function: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. Subunit: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Abundant in lymphoid tissues. Post-translational modifications: Glycosylation is tissue specific. Sialylation of N-glycans at Asn-93 in brain and at Asn-42, Asn-93 and Asn-117 in thymus. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P04216 Gene ID: 7070 Database links: Entrez Gene: 7070 Human Entrez Gene: 21838 Mouse Omim: 188230 Human SwissProt: P04216 Human SwissProt: P01831 Mouse Unigene: 644697 Human Unigene: 3951 Mouse Unigene: 108198 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Thymus tissue lysates
Primary: Anti-CD90/Thy-1 (bsm-52869R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 25 kDa
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy-1 Monoclonal Antibody, Unconjugated(bsm-52869R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy-1 Monoclonal Antibody, Unconjugated(bsm-52869R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated(bsm-52869R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated(bsm-52869R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated(bsm-52869R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated(bsm-52869R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
