| 產(chǎn)品編號 | bs-14265R |
| 英文名稱 | Deltex Rabbit pAb |
| 中文名稱 | E3泛素蛋白連接酶抗體DTX1抗體 |
| 別 名 | Deltex homolog 1(Drosophila); Deltex protein 1; Deltex-1; Deltex1; Dtx1; E3 ubiquitin protein ligase DTX1; FXI-T1; Fxit 1; Fxit1; DTX1_HUMAN; hDTX1; hDx 1; mDTX1; Protein deltex 1. |
| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學 轉(zhuǎn)運蛋白 表觀遺傳學 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Pig,Sheep,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 67 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Deltex: 351-450/620 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Studies in Drosophila have identified this gene as encoding a positive regulator of the Notch-signaling pathway. The human gene encodes a protein of unknown function; however, it may play a role in basic helix-loop-helix transcription factor activity. [provided by RefSeq, Jul 2008] Function: Deltex is a RING finger ubiquitin ligase which is conserved from Drosophila to Humans and is a regulator of Notch signaling pathway. Deltex regulates both B-cell lineage and splenic marginal-zone B-cell commitment. Deltex is implicated in neurogenesis, lymphogenesis and myogenesis as well as marginal zone B cell differentiation. Subunit: Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1 via its N-terminus region and EIF3F, the interaction is required for NOTCH1 deubiquitination. Interacts with EP300. Forms a heterodimer with BBAP; the heterodimerization leading to an increase of in vitro ubiquitin ligase activity. Interacts with ITCH. Subcellular Location: Cytoplasm and Nucleus. Note: Predominantly cytoplasmic, partially nuclear. Tissue Specificity: Widely expressed. Strongly expressed in blood vessel. Also expressed in embryonic nervous system, pancreas, lung, adrenal gland, digestive tube and muscles. Expressed in MZB cells and developing B- and T-cells. Post-translational modifications: Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH. Similarity: Belongs to the Deltex family. Contains 1 RING-type zinc finger. Contains 2 WWE domains. SWISS: Q86Y01 Gene ID: 1840 Database links: Entrez Gene: 1840 Human Entrez Gene: 14357 Mouse Omim: 602582 Human SwissProt: Q86Y01 Human SwissProt: Q61010 Mouse |
| 產(chǎn)品圖片 |
Sample:
Kidney (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Deltex (bs-14265R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kD
Observed band size: 67 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Deltex) Polyclonal Antibody, Unconjugated (bs-14265R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
