| 產(chǎn)品編號 | bs-1994R |
| 英文名稱 | MUC4 Rabbit pAb |
| 中文名稱 | 粘蛋白4抗體 |
| 別 名 | Mucin4; Mucin-4; Mucin 4; MUC-4; Ascites sialoglycoprotein; ASGP; Pancreatic adenocarcinoma mucin; Testis mucin; Tracheobronchial mucin; Mucin-4 alpha chain; Ascites sialoglycoprotein 1; ASGP-1; Mucin-4 beta chain; Ascites sialoglycoprotein 2; ASGP-2; Muc |
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Specific References (1) | bs-1994R has been referenced in 1 publications.
[IF=4.545] Chae Eun Kim. et al. Cevimeline-induced anti-inflammatory effect through upregulations of mucins in the ocular surface of a dry eye mouse model. Biomed Pharmacother. 2021 Jul;139:111571 IF ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 微生物學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Cow,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 80 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Mucin-4 beta chain: 1501-1613/2169 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq]. Function: May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Subunit: A heterodimeric complex, composed of a mucin-4 alpha chain and a cysteine-rich transmembrane mucin-4 beta chain. Mucin-4 beta chain interacts with ERBB2 via the EGF-like domain 1. In nonpolarized cells, associates with ERBB2 and ERBB3. Subcellular Location: Membrane; Single-pass membrane protein (Potential). Secreted. Note=Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing. Mucin-4 beta chain: Cell membrane; Single-pass membrane protein. Mucin-4 alpha chain: Secreted. Isoform 3, 17: Cell membrane; Single-pass membrane protein. Isoform 11, 15: Secreted. Tissue Specificity: Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines. Post-translational modifications: Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain. mucrnin-4 alpha chain is highly O-glycosylated. mucin-4 beta chain is predominantly N-glycosylated. Similarity: Contains 1 AMOP domain. Contains 2 EGF-like domains. Contains 1 NIDO domain. Contains 1 VWFD domain. SWISS: Q99102 Gene ID: 4585 Database links: Entrez Gene: 4585 Human Entrez Gene: 140474 Mouse Omim: 158372 Human SwissProt: Q99102 Human SwissProt: Q8JZM8 Mouse Unigene: 369646 Human Unigene: 214599 Mouse 腫瘤生物標(biāo)志Tumor biomarkers |
| 產(chǎn)品圖片 |
Sample:
Panc-1(Human) Cell Lysate at 30 ug
Lovo(Human) Cell Lysate at 30 ug
Thymus (Mouse) Lysate at 40 ug
MKN45(Human) Cell Lysate at 30 ug
Primary: Anti-MUC4?(bs-1994R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 120 kD
Paraformaldehyde-fixed, paraffin embedded (Human oral squamous cell carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Muc4) Polyclonal Antibody, Unconjugated (bs-1994R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human lung carcinom); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Muc4) Polyclonal Antibody, Unconjugated (bs-1994R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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