| 產(chǎn)品編號 | bs-1383R |
| 英文名稱 | CD13 Rabbit pAb |
| 中文名稱 | CD13抗體 |
| 別 名 | ANPEN; Aminopeptidase N; Alanyl aminopeptidase; Alanyl membrane aminopeptidase; Aminopeptidase M; Aminopeptidase N; ANPEP; APN; CD 13; CD13 antigen; gp150; hAPN; Lap 1; Lap1; Alanyl(membrane) aminopeptidase; AMPN_HUMAN; AP M; AP N; AP-M; AP-N; LAP 1; LAP1 氨肽酶N; |
 |
Specific References (1) | bs-1383R has been referenced in 1 publications.
[IF=0.88] Erikci, Acelya, Gulberk Ucar, and Samiye Yabanoglu-Ciftci. "Role of serotonin in the regulation of renal proximal tubular epithelial cells." Renal failure 38.7 (2016): 1141-1150. IF(ICC) ; Mouse.
|
| 研究領域 | 腫瘤 細胞生物 干細胞 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 109 kDa |
| 檢測分子量 | 150-170 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD13: 344-444/444 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. This membrane-bound zinc metalloprotease is known to serve as a receptor for the HCoV-229E alphacoronavirus as well as other non-human coronaviruses. This gene has also been shown to promote angiogenesis, tumor growth, and metastasis and defects in this gene are associated with various types of leukemia and lymphoma. [provided by RefSeq, Apr 2020] Function: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Subunit: Homodimer. Interacts with the S1 domain of HCoV-229E spike protein. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Cytoplasm, cytosol (Potential). Note=A soluble form has also been detected. Tissue Specificity: Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients. Post-translational modifications: Sulfated. N- and O-glycosylated. May undergo proteolysis and give rise to a soluble form. Similarity: Belongs to the peptidase M1 family. SWISS: P15144 Gene ID: 290 Database links: Entrez Gene: 290 Human Entrez Gene: 16790 Mouse Omim: 151530 Human SwissProt: P15144 Human SwissProt: P97449 Mouse Unigene: 1239 Human Unigene: 4487 Mouse Unigene: 11132 Rat Unigene: 179371 Rat 氨肽酶N(又稱CD13)是氨肽酶系列的一種，氨肽酶是從蛋白質(zhì)多肽鏈氨基端催化降解氨基酸殘基的水解蛋白酶。是多種冠狀病毒的受體，目前在腫瘤侵襲、轉(zhuǎn)移、免疫調(diào)節(jié)和病毒感染等多方面受人們的關注.它在腫瘤細胞表面高水平表達,對腫瘤細胞外基底膜起到降解作用引發(fā)腫瘤的侵襲和轉(zhuǎn)移。主要分布于小腸和腎臟，巨噬細胞、粒細胞和中樞神經(jīng)系統(tǒng)的突觸膜也表達CD13. |
| 產(chǎn)品圖片 |
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD13/APN/ANPEN Polyclonal Antibody, Unconjugated(bs-1383R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD13/APN/ANPEN Polyclonal Antibody, Unconjugated(bs-1383R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Black line : Positive blank control U937); Negative blank control (A431）
Green line : Primary Antibody (Rabbit Anti- CD13 antibody (bs-1383R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF647)
U937（Positive）and A431 Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD13 Antibody(bs-1383R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control: U937 (blue). Primary Antibody:Rabbit Anti- CD13 antibody(bs-1383R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-1383R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
